How to Reconstitute and Use Botulax 100U: Complete Clinical Guide

Botulax 100U by Hugel Inc. is one of the most widely used Korean botulinum toxin type A products in European aesthetic clinics. Correct reconstitution and handling are essential to ensure both product efficacy and patient safety. This guide provides a complete clinical reference for aesthetic practitioners incorporating Botulax 100U into their practice.

Understanding Botulax 100U

Botulax 100U is a lyophilised (freeze-dried) preparation of Clostridium botulinum toxin type A, containing 100 units of biological activity per vial. The excipients include human serum albumin and sodium chloride. Like all botulinum toxin products, Botulax must be reconstituted prior to injection with preservative-free saline.

Reconstitution Protocol

Proper reconstitution is the foundation of consistent clinical results. The dilution volume determines the concentration, and therefore the precision with which you can dose each treatment area. Most European practitioners use 2.5 ml as their standard dilution, yielding 4 units per 0.1 ml, which provides excellent precision for facial work.

Step-by-Step Reconstitution

1. 1
   Gather materialsPreservative-free 0.9% sodium chloride, 3 ml reconstitution syringe, 21G needle for reconstitution, 30–32G needles for injection, alcohol wipes, gloves.
2. 2
   Temperature equilibrationAllow Botulax vial to reach room temperature (5–10 min from fridge). Clean vial stopper with alcohol wipe.
3. 3
   Draw diluentAspirate the desired saline volume (typically 2.5 ml) into reconstitution syringe.
4. 4
   Gentle reconstitutionInsert needle into vial, inject saline slowly down the vial wall. The vacuum assists. Do NOT shake — gentle swirl only. Solution should be clear and colourless.
5. 5
   Label immediatelyDate, time, concentration (e.g. "4U/0.1ml"), initials. Refrigerate until use.
6. 6
   Draw treatment dosesUse 30–32G needle. Draw exact dose per treatment area into separate labelled syringes.

Clinical Applications and Dosing

Botulax 100U is suitable for all standard botulinum toxin treatment areas. The following dosing guidelines are based on published clinical protocols and widely adopted European aesthetic practice standards. Always adjust based on individual patient anatomy, muscle mass, and previous treatment history.

Injection Technique

Botulax behaves identically to other botulinum toxin type A products in terms of diffusion radius and injection technique requirements. Standard principles apply: inject into the belly of the target muscle, avoid vascular structures, use the minimal effective dose, and maintain at least 1 cm distance from the orbital rim for glabellar injections.

For perioral and lower face treatments, inject superficially to target the orbicularis oris and depressor muscles. Masseter injections should target the inferior third of the masseter muscle belly, avoiding the parotid gland and facial nerve branches.

Post-injection instructions for patients: avoid lying down for 4 hours, avoid vigorous exercise for 24 hours, do not massage the treated areas, avoid excessive heat. Review at 2 weeks for touch-up assessment if required.

Comparison with Other Korean Botulinum Toxins

Botulax is one of several high-quality Korean botulinum toxin products available through Korean Fillers EU. If you are considering alternatives for different clinical contexts, see our complete Korean botulinum toxin comparison guide. For a liquid-form toxin that requires no reconstitution, Innotox 50U is an excellent option.